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. 2014 Mar 7;7:15. doi: 10.3389/fnmol.2014.00015

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Copyright © 2014 Ghezzi, Liebeskind, Thompson, Atkinson and Zakon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Targeted RNAi knockdown of na or CG33988 expression disrupts diurnal control of locomotor behavior. UAS-responsive RNAi against na or CG33988 were pan-neurally driven using the elav-Gal4 driver. Control flies carry the elav-Gal4 driver in the w¹¹¹⁸ background used for the UAS-RNAi lines, but lacked the UAS-RNAi transgene. The locomotor activity of each genotype was monitored for a 7-day period and daily averages plotted in 30-minute bins. White bar at the X-axis indicates day period, black bar indicates night period. Asterisks indicate statistically significant difference between the induced-RNAi groups and the control groups for the individual 30-min bins (^*P < 0.05, repeated measures Two-Way ANOVA).