Figure 7. Antagonists of TrkC receptor modulate ligand-dependent signaling pathways in primary neuronal cultures.

Primary neuronal cultures were prepared from the cortex of embryonic day 14 mice (ed 14). (A) representative picture of a culture. (B) TrkC is detected in western blots of lysates obtained from these neurons, using anti-TrkC mAb 2B7. (C) Treatment with NT-3 (2 nM for 20 min) induces biochemical signals (p-TrkC, p-PLCγ, p-AKT) that are inhibited by co-treatment with compounds 1a or 3f (10 µM). In the absence of NT-3, the compounds did not have an effect on signals. Control β-tubulin III is loading standard.