Figure 9.

Intracellular bacteria in spleen and bone marrow cells. The levels of intracellular Burkholderia pseudomallei in the spleen (A) and bone marrow (B) were measured in the mice infected with NK19_mouse (white circles) and NK19_soil (gray circles) on Day 2, Day 6, and Day 14 post-infection. Only one point (Day 2 post-infection) is shown for the mice infected with NK19_mouse because all the mice died by d 4 post-infection. Fourteen days after infection with NK19_soil, the infected mice were reinfected with B. pseudomallei (NK19_mouse, white triangles; NKcd_mouse, white squares; NK19_soil, gray triangles; and NKcd_soil, gray squares). The data are not shown for another experiment (primary infection with NKcd_mouse versus NKcd_soil) because the levels of intracellular bacteria for each condition were similar to those shown (infection with NK19_mouse versus NK19_soil). The limit of detection for intracellular bacteria was 10 cfu/10⁶ cells. The symbol (*) signifies P < 0.05.