Table 2.
Diagnosis of paucibacillary (PB) patients by real-time polymerase chain reaction (PCR) and nested PCR tests
| Classification | No. | Real-time PCR | BI | PCR vs. BI | Nested PCR | Two kinds of PCR | ||
|---|---|---|---|---|---|---|---|---|
| Ct number* | Copy number | Positive (%) | Positive (%) | P value | Positive (%) | P value | ||
| LL | 9 | 24.9 ± 3.89 | 1.2 × 106 ± 3.3 × 106 | 9/9 (100) | 9/9 (100) | P = 1 | 9/9 (100) | P = 1 |
| BL | 10 | 27 ± 4.62 | 2.6 × 105 ± 4.1 × 105 | 10/10 (100) | 10/10 (100) | P = 1 | 10/10 (100) | P = 1 |
| BB | 10 | 30.8 ± 5.05 | 3.4 × 105 ± 1.0 × 106 | 10/10 (100) | 10/10 (100) | P = 1 | 10/10 (100) | P = 1 |
| BT | 8 | 32.6 ± 2.33 | 1.2 × 103 ± 1.8 × 103 | 8/8 (100) | 8/8 (100) | P = 1 | 8/8 (100) | P = 1 |
| TT | 8 | 34.6 ± 1.82 | 2.3 × 102 ± 3.2 × 102 | 4/8 (50) | 0/8 (−) | P = 0.04 | 2/8 (25) | P = 0.24 |
| BL 2008 year | 7 | 31.0 ± 5.16 | 4.7 × 105 ± 1.2 × 106 | 8/8 (100) | 8/8 (100) | P = 1 | 7/8 (88) | P = 0.5 |
| BL 2013 year | 8 | 27.4 ± 4.98 | 9.5 × 105 ± 2.3 × 106 | 10/10 (100) | 10/10 (100) | P = 1 | 10/10 (100) | P = 1 |
| Tinea versicolor | 5 | − | − | 0/5 | 0/5 | P = 1 | ||
| Psoriasis | 5 | − | − | 0/5 | 0/5 | P = 1 | ||
| Pityriasis rosea | 5 | − | − | 0/5 | 0/5 | P = 1 | ||
| Tinea corporis | 5 | − | − | 0/5 | 1/5 (20) | P = 0.5 | ||
| Multiple neurofibromatosis | 2 | − | − | 0/2 | 0/2 | P = 1 | ||
| Xanthomatosis | 2 | 34.6 ± 1.82 | 101 | 1/2 (50) | 0/2 | P = 0.5 | ||
| Scleroderma | 5 | − | − | 0/5 | 1/5 (20) | P = 0.5 | ||
| Normal person | 3 | − | − | 0/3 | 0/3 | P = 1 | ||
*
Threshold cycle [CT] values of ≤ 35. CT values < 35 were considered positive.
BI = bacterial index; LL = lepromatous leprosy; BL = borderline-lepromatous; BB = borderline-borderline; BT = borderline tuberculoid; TT = tuberculoid.