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Amount of antivenom protein in the experimental condition. The protein concentrations of all antivenom samples were determined by the BCA method. Based on the protein concentration of each antivenom and the specific volume used in the incubation with NHS, the amount of antivenom protein in each incubation was calculated. The results are expressed as the amount of antivenom protein (micrograms) per 200 μL NHS and represent the mean of two independent experiments.
