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. 2014 Mar 6;9(3):e89614. doi: 10.1371/journal.pone.0089614

Figure 3. S. aureus and P. aeruginosa planktonic and sessile cells in single and dual cultures.

Figure 3

Bacteria were grown overnight in 96-well flat-bottom microtiter plates in NB medium at 37°C either individually cultured or co-cultured at a 1∶1 ratio. CFU counts were determined in both planktonic and sessile fractions. Panel A: planktonic (left) and sessile (right) cells of S. aureus strain Newman in pure culture and in co-culture with P. aeruginosa strains PA14, AA2 and AA43. Statistically significant differences are referred to Newman in pure culture. Panel B: planktonic (left) and sessile (right) cells of P. aeruginosa strains PA14, AA2 and AA43 in pure culture and in co-culture with S. aureus strain Newman. The values represent the means of three independent experiments, and the bars indicate standard deviation. Statistically significant differences in non-parametric Mann–Whitney test are indicated by symbols when present: **: p<0.01; ***: p<0.001.