Table 2. IgE binding epitopes detected. The minimum unit necessary for IgE recognition is shown in bold and the residues forming alpha helix are underlined.

Location of peptides	Epitope	Microarray peptide	SPOTs peptide	Amino acid sequence	Sera
	E1	2		4-PPPPPFLAGAPQDVV KAFFE-23	1
		3		7-PPFLAGAPQDVV KAFFELLK-26
α helix			3	7-PPFLAGAPQDVV -18
H1(15–27)	E2		5	13-APQDVVKAFFEL-24	2
		7		19-KAFFEL LKKDETKTDPEIEK-38
	E3	8		22-FELLKKDETKTDPEI EKDLD-41	3
			9	25-LKKDETKTDPEI-36
			10	28-DETKTDPEIEKD-39
α helix			11	31-KTDPEI EKDLDA-42
H2 (33–47)	E4	11		31-KTDPEIEKDLDAWVDTL GGD-50	4
			13	37-EKDLDAWVDTL G-48
			14	40-LDAWVDTLGGDY-51
α helix	E5	14		40-LDAWVDTLGGDYKAKFETFK-59	5, 6, 7
H3 (51–77)
	E6	26		76-AVAKMTPEAKKADAELSKIA-95	5, 6, 8
			29	85-KKADAELSKIA E-96
		26		76-AVAKMTPEAKKADAELSKIA-95	5
α helix	E7	27		79-KMTPEAKKADAELSKIAEDD-98
H4 (82–96)		28		82-PEAKKADAELSKIAEDDSLN-101
		29		85-KKADAE LSKIAEDDSLNGIQ-104
			27	79-KMTPEAKKADAE-90
			29	85-KKADAE LSKIAE-96
	E8	31		91-LSKIAEDDSLNGIQKAQKIQ-110	4
			31	91-LSKIAEDDSLNG-102
α helix	E9	33		97-DDSLNGIQKAQKIQAIYKTL-116	7
H5 (103–113)
α helix	E10	35		103-IQKAQKIQAIYKTLPQSVKD-122	3, 7, 9
H6 (118–125)	E11	37		109-IQAIYKTLPQSVKDELEKGI-128	2