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. 2014 Feb 4;106(3):687–695. doi: 10.1016/j.bpj.2013.12.035

Figure 3.

Figure 3

Indentation experiments on single vaults and histogram of the recovery time. (A and B) Images of a reclining particle (A) and a half-vault (B) before (upper) and after (middle) the FIC (lower). The forward curve (black) provides the elastic constant (slope of the initial linear part of the curve, green dashed line), the breaking force (maximum force reached, a), and the critical indentation (at a). The backward curve (gray) shows recovery during retraction of the tip. The same experiment performed on a half-vault. Shaded area in B represents the energy supplied to the vault during a FIC. Scale bar, 25 nm. (C) Histogram of the recovery time (RT) of both orientations. RT was classified as fast (RT < 650 ms), occurring during the retraction of the tip; medium (650 ms < RT < 2 s), occurring after the retraction of the tip but before the imaging of the particle; and slow (RT > 2 s), occurring during the imaging process. The histogram contains 260 FICs from 26 half-vaults (black) and 357 FICs from 28 reclining particles (red). Numbers of particles in the three RT ranges are shown above the bars. To see this figure in color, go online.