Mechanical fatigue of a half-vault. This particle was imaged along 37 frames at an imaging force of 65 pN (Movie S1). (A–C) Topographical images corresponding to frames 1, 16, and 35, respectively. Scale bar, 25 nm. (D) Evolution of the profile taken along the dotted line in frame 1. The profiles corresponding to frames 1, 16, and 35 are depicted in red, blue, and green, respectively. The rest of the profiles are depicted with dotted gray lines. (E) Sketch of the disruption suffered by the particle. To see this figure in color, go online.