Figure 4.

Fibroblast migration tracks measured after 1 day of wound infiltration. Migration tracks were measured in three gels per group, yielding a total of 568 cells tracked for long-axis restraint and 471 cells tracked for short-axis restraint. (A) For long-axis restraint, fibroblasts migrated preferentially toward the center of the wound (+x-direction). (B) For short-axis restraint, fibroblasts also migrated preferentially toward the center of the wound and migration tracks were aligned more strongly along the short axis (x axis). (C and D) The computational model reproduced the bulk of the fibroblast migration tracks for both mechanical conditions, except for the small subset of cells with highest net displacement.