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. 2014 Feb 28;13:73. doi: 10.1186/1475-2875-13-73

Table 1.

Set of primer sequences used to characterize gene polymorphisms by Plasmodium falciparum and P . vivax

Gene Codons Primer AT* (°C) PCR products (bp)
pfmdr1
86, 184
5′-GAGTTGAACAAAAAGAGTACCGCTGA-3′
55
512
5′-TTTTTCCGTTAATTTATGTTTGTGGTGTCA-3′
1043, 1042
5′-TGTCAAGCGGAGTTTTTGCATTTAGT-3′
299
5′-TGGTAGTTATGCTGGAAAATTAATGTCCT-3′
1246
5′-GGAGAAACAGGTAGTGGAAAATCAACTT-3′
302
5′-TTTGGAAGAGAAGATGCAACATTGGAA-3
pvmdr1
976
5′-ACTCACTTTATAGTGCTCTTCCTTGTG-3′
55
476
5′- GGACATCAACTTCCCGGCGT- 3′
pfcrt
72, 76
5′- acagATGGCTCACGTTTAGG -3′
55
162
    5′- TTTTGTAACATCCGAAACTCACA -3′    

*Annealing temperature.