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. 2014 Feb 21;444(4):473–479. doi: 10.1016/j.bbrc.2014.01.068

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© 2014 The Authors

This is an open access article under the CC BY NC ND license (https://creativecommons.org/licenses/by-nc-nd/3.0/).

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Fig. 1 — No early UB branching defects in Pkd1^−/− compared to Pkd1^+/+ and Pkd1^+/− explants. (A–E) Metanephric explants from embryos of 11.5 days were cultured for 3 days. (A) Staining for cytokeratin (green), marker of the UB epithelium in Pkd1^+/+, Pkd1^+/− and Pkd1^−/−renal explants shows similar UB elongation and branching. (B) Laminin staining (red), which marks the basement membranes of epithelializing structures, of the kidney rudiments isolated at day E11.5 and cultured for 3 days, does not show defects of early nephrogenesis. (C) Merge of cytokeratin and laminin. (D and E) Time-lapse video microscopy for up to 60 h of Pkd1^+/+(D) or Pkd1^−/−. (E) Hoxb7-GFP transgenic kidneys isolated at E11.5 show normal kinetics of UB branching and elongation of Pkd1^−/− explants. (F) Quantification of UB tips number of renal explants isolated at E11.5 and cultured for 3 days. Means ± SD are given; n.s.: no significant; n indicates the number of kidneys. Counts were performed on four independent litters. (Bar = 500 μm). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)