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. 2014 Feb 21;444(4):473–479. doi: 10.1016/j.bbrc.2014.01.068

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© 2014 The Authors

This is an open access article under the CC BY NC ND license (https://creativecommons.org/licenses/by-nc-nd/3.0/).

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Fig. 2 — Impaired nephrogenesis in Pkd1^−/− cultured kidneys. (A–F) Kidneys withdrawn at E14.5 and cultured for 72 h were stained for WT1 (red), marker of condensed mesenchyme (arrow) and glomeruli (arrowhead). Two different magnifications of Pkd1^+/+ (A and D), Pkd1^+/− (B and E) and Pkd1^−/− (C and F) revealed decreased glomeruli (arrowheads) and increased condensed mesenchyme (arrows) in Pkd1^−/− as compared to Pkd1^+/− and Pkd1^+/+ explants. (G and H) Co-staining of cytokeratin (green) and WT1 (red) showing more abundant glomeruli in Pkd1^+/+ kidneys (G) compared to the Pkd1^−/− explants (H), whereas there is more condensed mesenchyme in the mutant compared to the wt. (I) The number of the glomeruli in Pkd1^−/− kidneys is significantly impaired compared to the Pkd1^+/+ and Pkd1^+/− kidneys. Means ± SD are given; n.s.: no significant P > 0.05; ^∗∗∗P < 0.001. n indicated corresponds to the number of kidneys. Experiments were carried out on three independent litters. (A–C) Bar = 500 μm, (F–H) Bar = 200 μm). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)