Figure 6.

Loss of Argonaute2 during Insulin Resistance Inhibits Compensatory Proliferation

(A) Immunostaining of pancreatic sections from Ago2ob mice and ob/ob littermates with antibodies to insulin (green) and glucagon (red). Scale bars = 200 μm.

(B) β cell mass analysis of 10-week-old wild-type (WT), ob/ob, and Ago2ob mice (n = 3).

(C) Morphometric analysis of insulin+ and glucagon+ cells in 10-week-old WT, ob/ob, and Ago2ob (n = 4–5).

(D) Pancreatic weight in 10-week-old Ago2ob mice and ob/ob littermates (n = 4–5).

(E and F) Random blood glucose and plasma insulin levels of 10-week-old Ago2ob and littermate ob/ob mice (n = 4–5).

(G) Plasma insulin levels after glucose bolus on 10-week-old Ago2ob mice and ob/ob littermates (n = 4–5).

(H) Blood glucose levels during a GTT on 10-week-old Ago2ob mice and ob/ob littermates (n = 4–5).

(I) Blood glucose levels during an ITT on 10-week-old Ago2ob mice and ob/ob littermates (n = 4–5).

(J) Body weight of Ago2ob mice and ob/ob mice from 4–10 weeks of age (n = 4–5).

(K) Western blot analysis of Ago2 and Cadm1 in islets from 10-week-old Ago2ob mice and ob/ob littermates.

(L) Blood glucose levels during a GTT on 16-week-old βAgo2KO mice and littermates after 12 weeks on a HFD (n = 6).

(M) β cell mass analysis in 16-week-old βAgo2KO mice and littermates after 12 weeks on a HFD (n = 6).

(N and O) Morphometric analysis of insulin+, glucagon+, and BrdU+ cells in 16-week-old βAgo2KO mice and littermates after 12 weeks on a HFD (n = 6). Results presented as mean ± SEM. ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001.

See also Figure S6.