Figure 4.
Inducible Tregs interfere with the interactions between donor T cells and host DCs comparable to eTregs. (A-C) Intravital imaging of donor T cells (labeled with CMPTX) and host DCs of irradiated CD11c-DTR-EGFP mice in the presence of eTregs (Tcon:eTreg ratio of 3:2) or iTregs (Tcon:iTreg ratio of 1:1). (A) The time course of mean velocity of donor T cells in the presence of iTregs or eTregs. (B) The time course of the displacement ratio of donor T-cell movement in the presence of iTregs or eTregs. (C) Contact time between donor T cells and DCs with iTregs or eTregs at 5 or 8 hours (hr) posttransplant. (D-F) Intravital imaging of donor iTregs or eTregs (labeled with CMPTX) and host DCs in the presence of unlabeled donor T cells. (D) Still image from movies of iTregs (red) with DCs (green). (E) Comparison of mean velocity between iTregs and eTregs cells at various time points after transplant. (F) Contact time between iTregs–DC in comparison with contact time between eTregs and DCs at 2 different time points after transplant. The bars in all graphs represent median values. Asterisks indicate statistically significant differences (*P < .05) or P value is shown. Data are representative of 2 experiments with 1 to 2 mice per experiment.