Figure 9. Accelerated blood–brain barrier breakdown and microvascular reductions in pericyte-deficient APP^sw/0Pdgfrβ^+/−mice.

(a,b) Western blot analysis of serum IgG in capillary-depleted cortical homogenates relative to β-actin (a) and quantification of IgG extravascular levels (b) in 1-month-old Pdgfrβ^+/+, Pdgfrβ^+/−, APP^sw/0; Pdgfrβ^+/+ and APP^sw/0; Pdgfrβ^+/− mice. Full-size blots can be found in Supplementary Fig. S5. (c) Total length of lectin-positive capillary profiles in 1-month-old Pdgfrβ^+/+, Pdgfrβ^+/−, APP^sw/0; Pdgfrβ^+/+ and APP^sw/0; Pdgfrβ^+/− mice. In b, values are means±s.e.m., n=4 mice per group. In c, values are means±s.e.m., n=6 mice per group. P<0.05 by ANOVA followed by Tukey’s post-hoc tests. (d,e) IgG extravascular leakage (d, scale bar, 100 μm) and quantification of IgG extravascular deposits (e) in 9-month-old Pdgfrβ^+/+, Pdgfrβ^+/−, APP^sw/0; Pdgfrβ^+/+ and APP^sw/0; Pdgfrβ^+/− mice.(f) Total length of lectin-positive capillary profiles in 9-month-old Pdgfrβ^+/+, Pdgfrβ^+/−, APP^sw/0; Pdgfrβ^+/+ and APP^sw/0; Pdgfrβ^+/− mice. In e,f, values are means±s.e.m., n=6 mice per group. P<0.05 by ANOVA followed by Tukey’s post-hoc tests.