Skip to main content
. 2013 Dec 10;24(3):359–371. doi: 10.1038/cr.2013.162

Figure 5.

Figure 5

RACK1 interfered with TRAF2-mediated recruitment of the IKK complex. (A) 293T cells were treated with TNF (10 ng/ml) for 0 to 60 min; the lysate was immunoprecipitated with mouse anti-IKKα antibody. (B) 293T cells were transfected with control vectors or myc-RACK1 vectors, and 24 h later, the cells were treated with TNF (10 ng/ml) for the indicated time. The lysates were immunoprecipitated with mouse anti-IKKα antibody. (C) Knockdown of RACK1 enhanced the TRAF2-mediated recruitment of the IKK complex. (D) 293T cells were transfected with the indicated vectors, and 24 h later, the lysates were immunoprecipitated with mouse anti-HA antibody. (E) 293T cells were transfected with HA-IKKβ and Flag-TRAF2 vectors, and 24 h later, the lysates were incubated with purified GST protein (10 μg) or GST-RACK1 protein (10 μg). After 4 h of incubation, half of the lysate was immunoprecipitated with mouse anti-HA antibody, and the other half was immunoprecipitated with mouse anti-Flag antibody.