Fig. 4.

Triple Venn diagrams illustrating the relationship between the numbers of candidate disease-specific markers identified within the diseased spinal cord/CSF from various quantitative proteomic analyses. Overall there are 44 proteins in common including 6 well-validated markers of EAE: complement C3, fetuin A, hemopexin and ceruloplasmin, beta 2 microglobulin, and Ig gamma-2A chain C region. However, a large proportion of proteins identified in this study by MS¹- and MS²-based quantitation (exhibiting a ≥2-fold change in relative expression) that have yet to be linked to EAE or multiple sclerosis. Differences in the number of proteins observed between the three studies may be due the use of different rodent EAE models (MOG versus MBP-induced), varying capabilities of the mass spectrometry instrumentation and alternative methods of protein quantification.