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. 2013 Dec 20;13(3):679–700. doi: 10.1074/mcp.M113.033340

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Fig. 9. — Targeted mass spectrometry analysis of a subset of differentially abundant proteins identified within the membrane protein-enriched fraction of PBMCs isolated from EAE and sham-induced mice. Proteins validated to be up-regulated (A) and down-regulated (B) in EAE PBMCs or (C) “housekeeping” proteins (HSP70 and ALDH2) exhibiting similar levels of expression between sham and EAE mice Graphs are representative XICs for specific peptides derived from dysregulated proteins sampled from each group. Bar charts represent the average peak intensity (cps) for peptides quantified in EAE (red) and sham (green) mice (three biological replicates per group, two technical replicates per peptide). A Student's t test was performed on the peak areas of each quantified peptide to test for statistical differences between EAE and sham with p values ≤ 0.05 considered statistically significant. n.s refers to peptides that were analyzed by MRM but were deemed not statistically significant. * indicates that the t test p values for the peptide failed to reach statistical significance after multiple hypothesis testing.

Fig. 9. — Targeted mass spectrometry analysis of a subset of differentially abundant proteins identified within the membrane protein-enriched fraction of PBMCs isolated from EAE and sham-induced mice. Proteins validated to be up-regulated (A) and down-regulated (B) in EAE PBMCs or (C) “housekeeping” proteins (HSP70 and ALDH2) exhibiting similar levels of expression between sham and EAE mice Graphs are representative XICs for specific peptides derived from dysregulated proteins sampled from each group. Bar charts represent the average peak intensity (cps) for peptides quantified in EAE (red) and sham (green) mice (three biological replicates per group, two technical replicates per peptide). A Student's t test was performed on the peak areas of each quantified peptide to test for statistical differences between EAE and sham with p values ≤ 0.05 considered statistically significant. n.s refers to peptides that were analyzed by MRM but were deemed not statistically significant. * indicates that the t test p values for the peptide failed to reach statistical significance after multiple hypothesis testing.