Fig. 3.

Hyper-acetylation of H4 N-terminal domain in ΔTXR1 and ΔEZL2 cells. H4 N-terminal domains exhibited progressive increases from low degree to high degree of acetylation. Levels of the fully, tetra-acetylated peptide were dramatically increased in the HMT mutants. A, combinatorial acetylation patterns in H4 N-terminal domains. B, H4 N-terminal tetra-acetylation confirmed via tandem mass spectrum of 841.9559 Da precursor ion at 2 ppm (Z = 2+, Ion score = 51). Note that PTM combinations in the heatmap are averaged over charge states and tryptic cleavage sites.