Fig. 4.

Validation of histone hyper-acetylation by acid-urea gel electrophoresis. Increased levels of acetylation from low-degree to high-degree in H2A and H4 N-terminal domains were also demonstrated by acid-urea gel electrophoresis in the HMT mutants. Histone samples were treated with λ protein phosphatase to remove phosphorylations before loading onto the gel. Five micrograms of total protein (either H4 or H2A) were loaded onto a 15% acid urea gel and run at 100V for 30 min followed by 400V for 10 h. Note: ΔE2+T1 is the double knockout strain of EZL2 and TXR1.