Fig. 11.

Confirmation of ERβ–VCP interaction and subcellular expression of ERβ-interaction partners. A, confirmation of ERβ–VCP interaction in ventral hippocampus by immunoblot with corresponding input and nonspecific IgG control. B, representative immunoblots for nuclear and cytosolic ERβ, HSP70, GAPDH, VCP, HNRNP H, and ENO1 expression in ventral hippocampus normalized to β-actin. C, quantification of densitometric analysis of protein expression calculated from at least three independent experiments (n = 3). *Significant difference between groups (two-way ANOVA, p < 0.05).