Table III. Results for the construction of protein assembly starting from the unbound forms of the components.

PDB structure	Number of chains	Number of residues	Unbound forms	Structurally different predictions	Energy	RMSD
2e86	3	1011	1et5A x3	1	−694.70	0.52
1eer	3	620	1buy, 1ernA x2	9	−75.58	5.56
1gp2	3	764	1giaA, 1tbgA, 1tbgE	1	−279.38	3.57
1ado	4	482	1aldA x4	15	−324.79	2.79
1akj	4	615	2clrA, 2clrB, 1cd8A x2	5	−272.70	2.57
1z0k	4	482	2bmeA x2, 1yzmA x2	13	−104.58	3.07
1b0c	5	290	9ptiA x5	23	−109.12	5.15
1wnr	7	658	3nx6A x7	13	−426.80	4.60

Predicted PDB structures are given with their PDB I.D., number of chains, and number of residues. If an unbound structure is used twice, it is denoted by “x2.” RMSD was calculated compared to the PDB structures for all backbone atoms, and the energy value of an assembly is the summation of energy values calculated for the addition of each protein. These are the best RMSD representatives of the structurally clustered predictions.