Figure 1.
The mixed tumor model composed of EGFR-positive (A431) and EGFR-negative (Balb/DsRed) tumor cells. Pan-IR700 (green) was intravenously injected 1 d before PIT. Scattered Balb/DsRed tumor foci (red) were observed in A431 cells (Green) before PIT. After PIT, severe damage was shown only in EGFR-positive A431 tumors, and the proportion of Balb/DsRed increased with time after PIT. Balb/DsRed cells eventually almost replaced the entire tumor at 7 days after PIT. Histological evaluation before and after PIT. EGFR-targeted PIT induced selective intense necrosis of EGFR-positve A431 cells as indicated by hematoxylin and eosin (H&E) staining. In contrast, spindle shaped Balb/DsRed cells remain intact even after PIT.