Figure 4.

Survivin down-regulation is associated with abnormal cytokinesis in primary cells of renal epithelia and vascular endothelial cells. (a) To obtain the mechanistic insights of cytokinesis defect in renal epithelial cells, live-cell imaging analysis was performed. (b) A similar study was also done in vascular endothelial cells. Control and survivin knockdown cells were loaded with Hoechst dye to examine nuclear division (lower panels), while DIC images were used to study cytokinesis (top panels). Time stamps indicate hours and minutes as illustrated in Supplementary Materials, Movies S1–S4. Bar=50 μm. N≥3 for each group and treatment.