Supplementary Fig. 5.

SU6668 prevents Shh-induced osteogenic differentiation of C3H10T1/2 cells. (A) C3H10T1/2 cells were treated as indicated for 3 d. Alkaline phosphatase (AP) activity was measured and normalized with the amount of total protein. The normalized level of AP activity in the control sample treated with DMSO was set as 1. The normalized data are expressed as an average mean ± S.D. ***—p < 0.001, n = 4. (B, C) C3H10T1/2 cells were treated for 4 d. Whole cell extracts were subjected to WB analysis. The levels of the respective gene mRNA expression were measured by qPCR, normalized with Hprt mRNA expression levels and set as 1 in the samples treated with DMSO. The data are presented as an average mean ± S.D. *—p < 0.05, **—p < 0.01, ***—p < 0.001, n = 3.