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. 2014 Mar 6;9(3):e90235. doi: 10.1371/journal.pone.0090235

Table 1. Primers used to evaluate the expression of genes associated with stress and virulence in E. faecalis.

Gene Primer sequence (5′→3′) Amplicon length (bp)
dnaK F: CAGTTAACCCTGACGAAG; R: TGACAACGGTGTTACGTC 104 bp
groEL F: CTGTTTCAGTTGCAGCAC; R: CAAATCGGCGAAACAACG 109 bp
ctsR F: CGTTGGATGGTAAAACGTG; R: CGTCAGATTTAATCGAGGC 145 bp
clpP F: GCAATCACTGAGTTTGCC; R: AATCATCTCGCGGTGAAC 109 bp
clpC F: AATACGGGCAAAACGACG; R: AACCTGCTTTAGCACGTG 110 bp
clpE F: CCTAGTAAACCTTGACCAG; R: CTTTCTCGCCAAATGCAAC 125 bp
clpX F: TTCTCGGACTGCTTCATC; R: TAATAACGGGACCGTTCG 154 bp
gls24 F: TAACAGTCGATGGCGGCTTT; R: CAGCGACTTGTTTTTTACCAACTTC 105 bp
efaA F: TGGGACAGACCCTCACGAATA; R: CGCCTGTTTCTAAGTTCAAGCC 101 bp
ace F: GGCGACTCAACGTTTGAC; R: TCCAGCCAAATCGCCTAC 100 bp
gelE F: GGAACAGACTGCCGGTTTAG; R: TTCTGGATTAGATGCACCCG 103 bp
sprE F: CCTGTCTGCAAATGCAGAAG; R: CTGCCACTTCTTGTCTTCTG 101 bp
cylB F: ATTGAAGTACGTTGCGCAAG; R: CCCTTAGTTCTACTAGTGTAC 104 bp
23S F: CCTATCGGCCTCGGCTTAG; R: AGCGAAAGACAGGTGAGAATCC 101 bp