Figure 2. Effects of TL therapy combinations on NB tumor vascularization and perfusion.

A. Representative T₂W fast SE images (top) and the corresponding ΔSco₂ (middle row) and ΔSo₂ (bottom) maps of control (left), TL-118^1/4 (middle column) and TL^1/4 + Gem (right) treated tumors (Bar = 1 cm). B. Mean ΔSo₂ values of the tumor and liver-ROI's calculated for control (blue), TL-118^1/4 (green) and TL^1/4 + Gem (purple) treated mice. C. Representative histological slides immuno-stained with the smooth muscle marker α-SMA (upper-rows) and with the endothelial cell marker CD31 (lower-rows), of control (top), TL-118^1/4 (middle) and TL^1/4 + Gem (bottom) treated tumors. Photographs were taken from the peripheral (left column) and central (right column) regions (original magnification ×20) demonstrating the higher vascularity in tumor periphery. D. Quantification of α-SMA positive vessels/HPF was determined from the tumor center (dark) and peripheral (light) areas for each of the treatment groups. (Mean ± SE; n = 5–7 mice/group).