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. 2014 Mar 6;9(3):e90383. doi: 10.1371/journal.pone.0090383

Figure 5. Lack of bIGL expression in HAC/TKO calves by RT-PCR is shown.

Figure 5

PBMCs from five HAC/TKO calves (Calf 1-5) at birth were subjected to RT-PCR to confirm lack of bIGL expression. The primer pairs, bIgL-Ld-F1x bIgL-C-R and L003-F2 x L004-R2, were used to amplify VJ-rearranged bIGL and constant region IGLC (bCλ) genes, respectively. The primer pair, bIgκ-FR, was used to amplify VJ-rearranged bIGK gene. N, negative control; P, positive control.