Skip to main content
. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: J Neurochem. 2013 Nov 13;128(4):491–506. doi: 10.1111/jnc.12495

Figure 6.

Figure 6

Effects of time of day on FAAH activity and expression in the hypothalamus were determined. FAAH activity was measured as the conversion of AEA to ethanolamine in membranes harvested at the times indicated; Km and Vmax values were determined from isotherms created from 8 concentrations of AEA. Mean values of Km (A) and Vmax (B) are shown (n=4–5); vertical lines indicate SEM. (C) Western blot analyses were used to examine FAAH expression following separation of membrane proteins using gel electrophoresis. FAAH density was normalized to the density of GAPDH determined in the same sample. Each point is the mean of 4–6 separate tissues; vertical lines represent SEM. (D) mRNA for FAAH was determined in PFC harvested at the times shown using qPCR. Relative expression was calculated using GAPDH as a calibrator. Each point is the mean of 5–6 replicates, vertical lines represent SEM. For each graph, two cycles of the same data are displayed. * p<0.05 by Bonferroni’s t-test.