Table 3. Constitutive Promoters (Type-B Spacers) (Rightward transcription).
Map | Left Operon | Left Gene | D | RpoD | D | Right Gene | Right Operon | Promoter sequence |
0.37 | sokC | sokC | > | HNS | > | nhaA | nhaAR | TTAACC-17-TCTAAT |
0.61 | dapB | dapB | > | > | carA | carAB | TTGACT-17-CAGAAT | |
0.99 | uaaU | yaaU | > | > | kefF | kefFC | TTGACT-16-TATGAC | |
2.27 | (mraZ- -lpxC) | ftsZ | > | > | lpxC | lpxC | ||
2.76 | (pdhR- -lpd) | aceF | > | > | lpd | lpd | TTTAAA-17-TAAAAT | |
3.55 | hrpB | hrpB | > | > | mrcB | mrcB | TTGAGA-17-TGTAAC | |
3.61 | mrcB | mrcB | > | > | fhuA | fhuACDB | TTGCGA-18-TATTAT | |
3.81 | clcA | clcA | > | > | erpA | erpA | TAGATA-19-TAGAAT | |
4.33 | (bamA- -lpxA) | lpxD | > | > | fabZ | (bamA- --lpxA) | TCGCCA-15-TCTCAT | |
4.36 | (bamA- -lpxA) | fabZ | > | > | lpxA | (bamA- -lpxA) | Internal Promoter | |
5.41 | dinB-yafNOP | dinB | > | > | yafN | yafNOP | ||
5.62 | (proBC) | proA | > | > | thrW | thrW | TTGACG-15-TTTAAC | |
7.15 | betT | betT | > | HNS | > | yahA | yahA | TTGATC-16-TATAAT |
8.44 | yaiU | yaiU | > | HNS | > | yaiV* | yaiV | TTCACT-18-TTTAAT |
8.73 | yaiI | yaiI | > | HNS | > | aroL | aroL-yaiA-aroM | TCGAAA-17-TATGAT |
9.33 | (nrdR- -pgpA) | ribD | > | > | ribE | (nrdR- -pgpA) | Internal Promoter | |
9.79 | bloA | bolA | > | > | tig | tig | TCGACT-17-TATAAT | |
9.83 | tig | tig | > | > | clpP | clpPX | TTGAAA-17-CATAAC | |
10.08 | cof | cof | > | > | ybaO* | ybaO | TTGTCG-17-TAAAAT | |
10.70 | adk | adk | > | > | hemH | hemH | TTATCA-15-GATATT | |
11.26 | (ybbAP) | ybbP | > | HNS | > | rhsD | rhsDC-yibH | TTAATA-17-TGTAAT |
11.36 | (rhsD- -ylbH) | ylbH | > | > | ybbD | ybbD | ||
11.38 | ybbD | ybbD | > | > | ylbI | ylbI | TCGTCA-19-TAAAAT | |
12.23 | (renD-emrE) | emrE | > | HNS | > | ybcK | ybcK | GTGACC-17-TAAAAA |
12.24 | ybcK | ybcK | > | HNS | > | ybcL | ybcLM | GTGGCA-17-TACAAT |
12.29 | ybcLM | ybcL | > | > | ybcM | (ybcLM) | Internal Promoter | |
12.55 | tfaX | tfaX | > | HNS | > | appY* | appY | TTATCA-17-TTTAAT |
13.06 | sokE | sokE | > | > | hokE | hokE | ||
13.56 | (entcEBAH) | entH | > | > | cstA | cstA | TTTACA-15-TAAATT | |
14.15 | pagP | pagP | > | > | cspE | cspE | TGGACA-17-TGTACT | |
14.57 | ybeR-djiB | ybeR | > | > | djiB | (ybeR-djiB) | Internal Promoter | |
15.84 | (ybfOC) | ybfC | > | ybfQ | ybfQ | TTTTCA-17-AATACT | ||
16.61 | (mngAB) | mngB | > | HNS | > | cydA | cydAB | TCTACA-17-TATATT |
17.49 | (biobFCD) | bioD | > | > | uvrB | uvrB | TTGGCA-17-TAAAAT | |
17.64 | (moaABCDE) | moaE | > | > | ybhL | ybhL | TGCACA-17-TATCCT | |
17.65 | ybhL | ybhL | > | HNS | > | ybhM | ybhM | ACGACA-16-TATAAA |
19.21 | (ybjC- -ybjN) | nfsA | > | > | rimK | rimK-ybjN | ||
20.23 | (lolA-rarA) | rarA | > | > | serS | serS | TGGCCA-17-GATAAG | |
20.68 | (serC-aroA) | aroA | > | > | ycaL | ycaL | TTGATA-17-ATTAAT | |
23.37 | (efeOB) | efeB | > | HNS | > | phoH | phoH | TTTATA-16-TATATT |
24.80 | (rpmF- -fabHDG) | fabG | > | > | acpP | acpP-fabF | TTGCAA-16-TACACT | |
24.84 | (acpP-fabF) | fabF | > | > | pabC | pabCG- -ycfH | CTGCCA-15-GATAAG | |
25.12 | (hinT- -ycfP) | ycfP | > | HNS | > | ndh | ndh | CTCACA-17-AACAAT |
25.92 | (ymfTL- -ymfS) | ymfL | > | > | ymfM | (ymfTL- -ymfS) | Internal Promoter | |
26.07 | pinE | pinE | > | > | mcrA | mcrA | TTGTCG-17-ATTAAT | |
26.19 | ycgZ- -ymgC | ycgZ | > | > | ymgA | (ycgZ- -ymgC) | Internal Promoter | |
26.20 | (ycgZ- -ymgC) | ariR | > | > | ymgC | (ycgZ- -ymgC) | Internal Promoter | |
26.22 | (ycgZ- -ymgC) | ymgC | > | HNS | > | ycgG | ycgG | TTGACG-19-TATTTT |
26.27 | ymgF | ymgF | > | HNS | > | ycgH | ycgHI | TTGACA-19-TATAAG |
28.00 | ychE | ychE | > | HNS | > | oppA | oppABCDF | TTAACA-17-AAGAAT |
28.51 | (yciVOQ) | yciO | > | > | yciQ | (yciVOQ) | Internal Promoter | |
28.71 | topA | topA | > | HNS | > | cysB* | cysB | TTCACA-15-TATAAA |
28.84 | pgpB | pgpB | > | > | yciS | yciSM | TTGATT-18-AATCAT | |
31.32 | (paaAB- -GHIJK) | paaB | > | > | paaC | (paaAB- -GHIJK) | Internal Promoter | |
31.44 | (paaAB- -GHIJK) | paaJ | > | > | paaK | (paaAB- -GHIJK) | Internal Promoter | |
31.63 | insI-2 | insI-2 | > | > | ydbC | ydbC | TTAACA-17-TCGAAT | |
32.89 | yncH | yncH | > | HNS | > | rhsE | rhsE | TTGACT-17-TATTAC |
34.53 | (lsrACD- -tam) | lsrD | > | > | lsrB | (lsrACD- -tam) | Internal Promoter | |
35.16 | ydfK | ydfK | HNS | > | pinQ | pinQ | TGTACA-16-AATAAT | |
35.51 | dicF | dicF | > | > | dicB | dicB- -insD-intQ | ||
35.98 | ynfM | ynfM | > | HNS | > | asr | asr | GTCACA-18-TGTAAT |
36.26 | (rstAB) | rstB | > | > | tus | tus | TGGTCA-17-TATAAA | |
37.36 | ydhO | ydhO | > | > | sodB | sodB | TTGCTA-16-AATAAG | |
37.49 | ydhC | ydhC | > | > | cfa | cfa | CTAACA-17-TGAAAT | |
38.12 | rprA | rprA | > | > | ydiL | ydiL | CTGATA-15-TATTGT | |
38.53 | aroH | aroH | > | HNS | > | ydiE | ydiE | TTGATA-16-TATCAA |
38.97 | yniC | yniC | > | > | ydjM | ydjM | CTGAAA-17-ATTAAT | |
38.99 | ydjM | ydjM | > | > | ydjN | ydjN | ATGACT-16-AATAAT | |
39.26 | nadE | nadE | > | > | cho | cho | TTGTCA-15-TTTAAT | |
41.46 | holE | holE | > | > | yobB | yobB-exoX | TATACA-16-CATAAC | |
43.96 | (yedVZ) | yedZ | > | > | zinT | zinT | TTGTCA-18-AATAAT | |
44.29 | amn | amn | > | HNS | > | yeeN | yeeN | TAGACG-18-TATAAT |
44.60 | yeeP | yeeP | > | > | isrC | isrC | TTGTCC-17-TAGAAT | |
44.60 | isrC | isrC | > | > | flu | flu | ||
46.62 | (mdtA- -baeSR) | baeR | > | > | yegP | yegP | CTGGCA-17-CATACT | |
48.45 | yeiH | yeiH | > | > | nfo | nfo | ||
48.47 | nfo | nfo | > | > | yeiI | yeiI | ||
49.23 | (yejLM) | yejM | > | > | proL | proL | TTGCAA-16-TAGTAT | |
49.61 | yojO | yojO | > | HNS | > | eco | eco | GCGACA-15-TATAAA |
51.29 | rbn | rbn | > | > | elaD | elaD | TTAAAA-18-TGTTAT | |
54.54 | cysZ | cysZ | > | > | cysK | cysK | ATGTCA-16-TATAGA | |
55.21 | (yffOP) | yffP | > | > | yffQ | yffQR | CTCACA-16-TATCAC | |
55.23 | (yffQR) | yffR | > | > | yffS | yffS | ||
56.02 | bcp | bcp | > | > | hyfA | hyfAB- -GHIJR | ATGACC-17-CAGAAT | |
56.02 | hyfAB- -GHIJR | hyfA | > | > | hyfB | (hyfAB- -GHIJR) | Internal Promoter | |
57.13 | sseA | sseA | > | HNS | > | ryfA | ryfA | TTGTCA-16-TATTGT |
58.93 | bamD | bamD | > | > | raiA | raiA | CTGTCA-18-TTTAGT | |
59.27 | nadK | nadK | > | > | recN | recN | TTTACG-17-TATAAA | |
59.35 | smpB | smpB | > | > | ssrA | ssrA | TGGTCA-18-TATACT | |
59.59 | (rnlAB) | rnlB | > | yfjP | yfjPQ | TTGAAA-15-TATCAT | ||
59.79 | yfjW | yfjW | > | > | yfjX | yfjXYJZ-ypjF | TTGGCA-19-TATAAA | |
59.82 | (yfjXYJZF) | ypjF | > | > | psaA | psaA | CTGACA-15-AACAAT | |
60.31 | ygaM | ygaM | > | > | nrdH | nrdHIEF | TCAACA-18-TATCAT | |
60.46 | (proVWX) | proW | > | > | proX | (proVWX) | TTATCA-16-AATAAC | |
60.51 | (ygaXY) | ygaY | > | > | ygaZ | ygaZH | TTAAGA-17-TATAAT | |
60.92 | (srlAEBD) | srlD | > | > | gutM* | gutM-srlRQ | ||
61.59 | mutS | mutS | > | HNS | > | pphB | pphB | TTAACG-17-TAAAAA |
64.43 | ygeF | ygeF | > | HNS | > | ygeG | ygeG | TTTAAA-17-TATCAA |
64.49 | ygeI | ygeI | > | > | pbl | pbi | TTGACC-16-GATACT | |
65.32 | yqfG | yqfG | > | > | idi | idi | TTGTCG-18-AATCAT | |
65.82 | zapA | zapA | > | > | ssrS | ssrS-fau | ||
65.83 | (ssrS-fau) | fau | > | > | sibC | sibC | TTGACA-15-CCTAAT | |
66.49 | metK | metK | > | HNS | > | galP | galP | ATAACA-18-TATAAC |
67.93 | metC | metC | > | > | yghB | yghB | TGGACA-15-TATTGT | |
68.61 | yqiC | yqiC | > | HNS | > | ygiL | ygiL | TCGATA-17-TATAAA |
69.10 | (ttdABT) | ttdB | > | > | ttdT | (ttdABT) | Internal Promoter | |
69.42 | ebgR | ebgR | > | > | ebgA | ebgAC | TTGCCG-15-TATTTT | |
69.55 | ygjJK | ygjJ | > | > | ygjK | (ygjJK) | Internal Promoter | |
69.79 | aix | alx | > | > | sstT | sstT | CTGACC-17-TGTCAT | |
69.93 | exuT | exuT | > | > | exuR* | exuR | TTTTCA-16-TAAACT | |
69.96 | exuR | exuR | > | > | yqjA | yqjA-mzrA | TTGTCT-17-TATAAA | |
70.39 | tdcR | tdcR | > | > | yhaB | yhaBC | TTGATA-19-GAAAAT | |
70.79 | (agaS--agaBCDI) | agaI | > | HNS | > | yraH | yraHI | TTGATA-17-TCAAAT |
71.84 | ispB | ispB | > | > | sfsB* | sfsB | TTTAGA-18-TATAGT | |
72.93 | argR | argR | > | HNS | > | yhcN | yhcN | TTGAAA-18-AATAAC |
73.46 | (panf-prmA) | prmA | > | > | dusB | dusB-fis | GTGCCA-18-AAAAAT | |
75.03 | yheST | yheS | > | > | yheT | (yheST) | Internal Promoter | |
75.35 | (nirBDC-cysG) | cysG | > | HNS | > | yhfL | yhfL | TTAACG-19-TATAAT |
75.39 | yhfL | yhfL | > | > | frlA | frlABCDR | CTGACA-18-TTTAAT | |
76.26 | yhgF | yhgF | > | HNS | > | feoA | feoABC | TTATCA-15-TTTAAT |
77.16 | yhhZA | yhhZ | > | > | insA-6 | insAB-6 | TTGAAA-17-TTTAAT | |
77.20 | (insAB-6) | insB6 | HNS | > | yrhD | yrhD | TAGAGA-18-TATATT | |
78.71 | slp-dctR | slp | HNS | > | dctR | dctR | TTAATA-17-TATTAT | |
80.65 | yiaK | yiaK | > | > | yiaL | (yiaK- -sgbHUE) | Internal Promoter | |
81.13 | yibA | yibA | > | > | yibJ | yibJ | ||
81.15 | yibJ | yibJ | > | > | yibG | yibG | ||
81.20 | yibV | yibV | > | yibU | yibU | TTAACT-15-GATAAT | ||
81.79 | (rfaD-waaFCL) | waaC | > | > | waaL | (rfaD-waaFCL) | Internal Promoter | |
82.24 | yicC | yicC | > | > | dinD | dinD | GTGAGA-15-TATAAA | |
82.34 | dinD | dinD | > | > | yicG | yicG | TTATCA-16-AAAAAT | |
82.48 | xanP | xanP | > | > | yicH | yicH | ||
85.10 | ilvLXGMEDA | ilvL | > | > | ilvX | (ilvLXGMEDA) | Internal Promoter | |
85.43 | trxA | trxA | > | > | rhoL | rhoL-rho | TTGACT-17-TATTAA | |
85.74 | (rfe- -tffT- -rffM) | rffM | > | > | yifK | yifK | ATTACA-15-TTTAAT | |
87.25 | polA | polA | > | > | spf | spf | CTGTCA-17-TAGAAA | |
87.47 | typA | typA | > | > | yihL | yihLM | ||
88.49 | fieF | fieF | > | > | pfkA | pfkA | ||
90.08 | (rplKAJL-rpoBC) | rplL | > | > | rpoB | (rplKAJL-rpoBC) | TAGTCA-15-TGTAAG | |
91.98 | aphA | aphA | > | > | yibQ | yibQR | ||
92.52 | (nrfABCDEFG) | nrfG | > | HNS | > | gltP | gltP | ATGCCA-18-TATTAT |
94.29 | ecnB | ecnB | > | > | sugE | sugE | TTGAAA-16-CAAAAT | |
94.89 | yjeT | yjeT | > | > | purA | purA | CTGAAA-19-TTTAAG | |
95.01 | (nsrR-rnr- -yjfIJ) | rlmB | > | > | yjfI | (nsrR-rnr- -yjfIJ) | TTAATA-17-TGGAAT | |
95.35 | (rpsF-priB- -rplI) | rpsR | > | > | rplI | (rpsF-priB- -rplI) | Internal Promoter | |
97.06 | (yjhBC) | yjhC | > | > | ythA | ythA | ||
97.85 | fimB | fimB | > | HNS | > | fimE | fimE | TTGTAA-17-CATAAT |
98.12 | (uxuAB) | uxuB | > | > | uxuR* | uxuR | GTGCCA-17-TATAGT | |
98.51 | yjiS | yjiS | > | HNS | > | yjiT | yjiT | TTGAGA-18-TATAAA |
99.35 | osmY | osmY | > | > | ytjA | ytjA | ||
99.36 | ytjA | ytjA | > | > | yjjU | yjjU | ||
99.52 | (deoCABD) | deoA | > | > | deoB | (deoCABD) | Internal Promoter | |
99.57 | (deoCABD) | deoD | > | HNS | > | yjjJ* | yjjJ | TTTTCA-18-TCTATT |
99.57 | yjjJ | yjjJ | > | HNS | > | yjtD | yjtD | TTGTCG-17-AATTAT |
HNS | 154 (b) | 271 (a) | ||||||
38 | 9 TFs | 1.76 (a/b) | ||||||
25% | 17 essential | 62 Y-genes |
Among the total of 1,075 RpoD holoenzyme-binding sites, 154 are located within type-B spacers upstream of right-side genes and downstream of lest-side genes, indicating that these promoters direct rightward transcription. The genes and operons under the control of these 154 promoters were estimated, of which 18 represent putative internal promoters. A total of 9 genes encoding transcription factors are indicated by star symbols (*). The essential genes listed in the PEC database are underlined within the operons. The promoter sequence with complete match with the canonical promoter (see Fig. 4) is shown in bold and italic while the promoter sequence with 5-out of-6 match is shown in bold. The spacers including H-NS binding sites are marked as HNS in the spacer column. Total number of genes under the control of 154 promoters were 271 (1.76 gene per promoter). Among the total of 154 RpoD holoenyme-binding sites, 38 (25%) overlap with the H-NS-binding sites.