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. 2014 Mar 6;9(3):e90447. doi: 10.1371/journal.pone.0090447

Table 3. Constitutive Promoters (Type-B Spacers) (Rightward transcription).

Map Left Operon Left Gene D RpoD D Right Gene Right Operon Promoter sequence
0.37 sokC sokC > HNS > nhaA nhaAR TTAACC-17-TCTAAT
0.61 dapB dapB > > carA carAB TTGACT-17-CAGAAT
0.99 uaaU yaaU > > kefF kefFC TTGACT-16-TATGAC
2.27 (mraZ- -lpxC) ftsZ > > lpxC lpxC
2.76 (pdhR- -lpd) aceF > > lpd lpd TTTAAA-17-TAAAAT
3.55 hrpB hrpB > > mrcB mrcB TTGAGA-17-TGTAAC
3.61 mrcB mrcB > > fhuA fhuACDB TTGCGA-18-TATTAT
3.81 clcA clcA > > erpA erpA TAGATA-19-TAGAAT
4.33 (bamA- -lpxA) lpxD > > fabZ (bamA- --lpxA) TCGCCA-15-TCTCAT
4.36 (bamA- -lpxA) fabZ > > lpxA (bamA- -lpxA) Internal Promoter
5.41 dinB-yafNOP dinB > > yafN yafNOP
5.62 (proBC) proA > > thrW thrW TTGACG-15-TTTAAC
7.15 betT betT > HNS > yahA yahA TTGATC-16-TATAAT
8.44 yaiU yaiU > HNS > yaiV* yaiV TTCACT-18-TTTAAT
8.73 yaiI yaiI > HNS > aroL aroL-yaiA-aroM TCGAAA-17-TATGAT
9.33 (nrdR- -pgpA) ribD > > ribE (nrdR- -pgpA) Internal Promoter
9.79 bloA bolA > > tig tig TCGACT-17-TATAAT
9.83 tig tig > > clpP clpPX TTGAAA-17-CATAAC
10.08 cof cof > > ybaO* ybaO TTGTCG-17-TAAAAT
10.70 adk adk > > hemH hemH TTATCA-15-GATATT
11.26 (ybbAP) ybbP > HNS > rhsD rhsDC-yibH TTAATA-17-TGTAAT
11.36 (rhsD- -ylbH) ylbH > > ybbD ybbD
11.38 ybbD ybbD > > ylbI ylbI TCGTCA-19-TAAAAT
12.23 (renD-emrE) emrE > HNS > ybcK ybcK GTGACC-17-TAAAAA
12.24 ybcK ybcK > HNS > ybcL ybcLM GTGGCA-17-TACAAT
12.29 ybcLM ybcL > > ybcM (ybcLM) Internal Promoter
12.55 tfaX tfaX > HNS > appY* appY TTATCA-17-TTTAAT
13.06 sokE sokE > > hokE hokE
13.56 (entcEBAH) entH > > cstA cstA TTTACA-15-TAAATT
14.15 pagP pagP > > cspE cspE TGGACA-17-TGTACT
14.57 ybeR-djiB ybeR > > djiB (ybeR-djiB) Internal Promoter
15.84 (ybfOC) ybfC > ybfQ ybfQ TTTTCA-17-AATACT
16.61 (mngAB) mngB > HNS > cydA cydAB TCTACA-17-TATATT
17.49 (biobFCD) bioD > > uvrB uvrB TTGGCA-17-TAAAAT
17.64 (moaABCDE) moaE > > ybhL ybhL TGCACA-17-TATCCT
17.65 ybhL ybhL > HNS > ybhM ybhM ACGACA-16-TATAAA
19.21 (ybjC- -ybjN) nfsA > > rimK rimK-ybjN
20.23 (lolA-rarA) rarA > > serS serS TGGCCA-17-GATAAG
20.68 (serC-aroA) aroA > > ycaL ycaL TTGATA-17-ATTAAT
23.37 (efeOB) efeB > HNS > phoH phoH TTTATA-16-TATATT
24.80 (rpmF- -fabHDG) fabG > > acpP acpP-fabF TTGCAA-16-TACACT
24.84 (acpP-fabF) fabF > > pabC pabCG- -ycfH CTGCCA-15-GATAAG
25.12 (hinT- -ycfP) ycfP > HNS > ndh ndh CTCACA-17-AACAAT
25.92 (ymfTL- -ymfS) ymfL > > ymfM (ymfTL- -ymfS) Internal Promoter
26.07 pinE pinE > > mcrA mcrA TTGTCG-17-ATTAAT
26.19 ycgZ- -ymgC ycgZ > > ymgA (ycgZ- -ymgC) Internal Promoter
26.20 (ycgZ- -ymgC) ariR > > ymgC (ycgZ- -ymgC) Internal Promoter
26.22 (ycgZ- -ymgC) ymgC > HNS > ycgG ycgG TTGACG-19-TATTTT
26.27 ymgF ymgF > HNS > ycgH ycgHI TTGACA-19-TATAAG
28.00 ychE ychE > HNS > oppA oppABCDF TTAACA-17-AAGAAT
28.51 (yciVOQ) yciO > > yciQ (yciVOQ) Internal Promoter
28.71 topA topA > HNS > cysB* cysB TTCACA-15-TATAAA
28.84 pgpB pgpB > > yciS yciSM TTGATT-18-AATCAT
31.32 (paaAB- -GHIJK) paaB > > paaC (paaAB- -GHIJK) Internal Promoter
31.44 (paaAB- -GHIJK) paaJ > > paaK (paaAB- -GHIJK) Internal Promoter
31.63 insI-2 insI-2 > > ydbC ydbC TTAACA-17-TCGAAT
32.89 yncH yncH > HNS > rhsE rhsE TTGACT-17-TATTAC
34.53 (lsrACD- -tam) lsrD > > lsrB (lsrACD- -tam) Internal Promoter
35.16 ydfK ydfK HNS > pinQ pinQ TGTACA-16-AATAAT
35.51 dicF dicF > > dicB dicB- -insD-intQ
35.98 ynfM ynfM > HNS > asr asr GTCACA-18-TGTAAT
36.26 (rstAB) rstB > > tus tus TGGTCA-17-TATAAA
37.36 ydhO ydhO > > sodB sodB TTGCTA-16-AATAAG
37.49 ydhC ydhC > > cfa cfa CTAACA-17-TGAAAT
38.12 rprA rprA > > ydiL ydiL CTGATA-15-TATTGT
38.53 aroH aroH > HNS > ydiE ydiE TTGATA-16-TATCAA
38.97 yniC yniC > > ydjM ydjM CTGAAA-17-ATTAAT
38.99 ydjM ydjM > > ydjN ydjN ATGACT-16-AATAAT
39.26 nadE nadE > > cho cho TTGTCA-15-TTTAAT
41.46 holE holE > > yobB yobB-exoX TATACA-16-CATAAC
43.96 (yedVZ) yedZ > > zinT zinT TTGTCA-18-AATAAT
44.29 amn amn > HNS > yeeN yeeN TAGACG-18-TATAAT
44.60 yeeP yeeP > > isrC isrC TTGTCC-17-TAGAAT
44.60 isrC isrC > > flu flu
46.62 (mdtA- -baeSR) baeR > > yegP yegP CTGGCA-17-CATACT
48.45 yeiH yeiH > > nfo nfo
48.47 nfo nfo > > yeiI yeiI
49.23 (yejLM) yejM > > proL proL TTGCAA-16-TAGTAT
49.61 yojO yojO > HNS > eco eco GCGACA-15-TATAAA
51.29 rbn rbn > > elaD elaD TTAAAA-18-TGTTAT
54.54 cysZ cysZ > > cysK cysK ATGTCA-16-TATAGA
55.21 (yffOP) yffP > > yffQ yffQR CTCACA-16-TATCAC
55.23 (yffQR) yffR > > yffS yffS
56.02 bcp bcp > > hyfA hyfAB- -GHIJR ATGACC-17-CAGAAT
56.02 hyfAB- -GHIJR hyfA > > hyfB (hyfAB- -GHIJR) Internal Promoter
57.13 sseA sseA > HNS > ryfA ryfA TTGTCA-16-TATTGT
58.93 bamD bamD > > raiA raiA CTGTCA-18-TTTAGT
59.27 nadK nadK > > recN recN TTTACG-17-TATAAA
59.35 smpB smpB > > ssrA ssrA TGGTCA-18-TATACT
59.59 (rnlAB) rnlB > yfjP yfjPQ TTGAAA-15-TATCAT
59.79 yfjW yfjW > > yfjX yfjXYJZ-ypjF TTGGCA-19-TATAAA
59.82 (yfjXYJZF) ypjF > > psaA psaA CTGACA-15-AACAAT
60.31 ygaM ygaM > > nrdH nrdHIEF TCAACA-18-TATCAT
60.46 (proVWX) proW > > proX (proVWX) TTATCA-16-AATAAC
60.51 (ygaXY) ygaY > > ygaZ ygaZH TTAAGA-17-TATAAT
60.92 (srlAEBD) srlD > > gutM* gutM-srlRQ
61.59 mutS mutS > HNS > pphB pphB TTAACG-17-TAAAAA
64.43 ygeF ygeF > HNS > ygeG ygeG TTTAAA-17-TATCAA
64.49 ygeI ygeI > > pbl pbi TTGACC-16-GATACT
65.32 yqfG yqfG > > idi idi TTGTCG-18-AATCAT
65.82 zapA zapA > > ssrS ssrS-fau
65.83 (ssrS-fau) fau > > sibC sibC TTGACA-15-CCTAAT
66.49 metK metK > HNS > galP galP ATAACA-18-TATAAC
67.93 metC metC > > yghB yghB TGGACA-15-TATTGT
68.61 yqiC yqiC > HNS > ygiL ygiL TCGATA-17-TATAAA
69.10 (ttdABT) ttdB > > ttdT (ttdABT) Internal Promoter
69.42 ebgR ebgR > > ebgA ebgAC TTGCCG-15-TATTTT
69.55 ygjJK ygjJ > > ygjK (ygjJK) Internal Promoter
69.79 aix alx > > sstT sstT CTGACC-17-TGTCAT
69.93 exuT exuT > > exuR* exuR TTTTCA-16-TAAACT
69.96 exuR exuR > > yqjA yqjA-mzrA TTGTCT-17-TATAAA
70.39 tdcR tdcR > > yhaB yhaBC TTGATA-19-GAAAAT
70.79 (agaS--agaBCDI) agaI > HNS > yraH yraHI TTGATA-17-TCAAAT
71.84 ispB ispB > > sfsB* sfsB TTTAGA-18-TATAGT
72.93 argR argR > HNS > yhcN yhcN TTGAAA-18-AATAAC
73.46 (panf-prmA) prmA > > dusB dusB-fis GTGCCA-18-AAAAAT
75.03 yheST yheS > > yheT (yheST) Internal Promoter
75.35 (nirBDC-cysG) cysG > HNS > yhfL yhfL TTAACG-19-TATAAT
75.39 yhfL yhfL > > frlA frlABCDR CTGACA-18-TTTAAT
76.26 yhgF yhgF > HNS > feoA feoABC TTATCA-15-TTTAAT
77.16 yhhZA yhhZ > > insA-6 insAB-6 TTGAAA-17-TTTAAT
77.20 (insAB-6) insB6 HNS > yrhD yrhD TAGAGA-18-TATATT
78.71 slp-dctR slp HNS > dctR dctR TTAATA-17-TATTAT
80.65 yiaK yiaK > > yiaL (yiaK- -sgbHUE) Internal Promoter
81.13 yibA yibA > > yibJ yibJ
81.15 yibJ yibJ > > yibG yibG
81.20 yibV yibV > yibU yibU TTAACT-15-GATAAT
81.79 (rfaD-waaFCL) waaC > > waaL (rfaD-waaFCL) Internal Promoter
82.24 yicC yicC > > dinD dinD GTGAGA-15-TATAAA
82.34 dinD dinD > > yicG yicG TTATCA-16-AAAAAT
82.48 xanP xanP > > yicH yicH
85.10 ilvLXGMEDA ilvL > > ilvX (ilvLXGMEDA) Internal Promoter
85.43 trxA trxA > > rhoL rhoL-rho TTGACT-17-TATTAA
85.74 (rfe- -tffT- -rffM) rffM > > yifK yifK ATTACA-15-TTTAAT
87.25 polA polA > > spf spf CTGTCA-17-TAGAAA
87.47 typA typA > > yihL yihLM
88.49 fieF fieF > > pfkA pfkA
90.08 (rplKAJL-rpoBC) rplL > > rpoB (rplKAJL-rpoBC) TAGTCA-15-TGTAAG
91.98 aphA aphA > > yibQ yibQR
92.52 (nrfABCDEFG) nrfG > HNS > gltP gltP ATGCCA-18-TATTAT
94.29 ecnB ecnB > > sugE sugE TTGAAA-16-CAAAAT
94.89 yjeT yjeT > > purA purA CTGAAA-19-TTTAAG
95.01 (nsrR-rnr- -yjfIJ) rlmB > > yjfI (nsrR-rnr- -yjfIJ) TTAATA-17-TGGAAT
95.35 (rpsF-priB- -rplI) rpsR > > rplI (rpsF-priB- -rplI) Internal Promoter
97.06 (yjhBC) yjhC > > ythA ythA
97.85 fimB fimB > HNS > fimE fimE TTGTAA-17-CATAAT
98.12 (uxuAB) uxuB > > uxuR* uxuR GTGCCA-17-TATAGT
98.51 yjiS yjiS > HNS > yjiT yjiT TTGAGA-18-TATAAA
99.35 osmY osmY > > ytjA ytjA
99.36 ytjA ytjA > > yjjU yjjU
99.52 (deoCABD) deoA > > deoB (deoCABD) Internal Promoter
99.57 (deoCABD) deoD > HNS > yjjJ* yjjJ TTTTCA-18-TCTATT
99.57 yjjJ yjjJ > HNS > yjtD yjtD TTGTCG-17-AATTAT
HNS 154 (b) 271 (a)
38 9 TFs 1.76 (a/b)
25% 17 essential 62 Y-genes

Among the total of 1,075 RpoD holoenzyme-binding sites, 154 are located within type-B spacers upstream of right-side genes and downstream of lest-side genes, indicating that these promoters direct rightward transcription. The genes and operons under the control of these 154 promoters were estimated, of which 18 represent putative internal promoters. A total of 9 genes encoding transcription factors are indicated by star symbols (*). The essential genes listed in the PEC database are underlined within the operons. The promoter sequence with complete match with the canonical promoter (see Fig. 4) is shown in bold and italic while the promoter sequence with 5-out of-6 match is shown in bold. The spacers including H-NS binding sites are marked as HNS in the spacer column. Total number of genes under the control of 154 promoters were 271 (1.76 gene per promoter). Among the total of 154 RpoD holoenyme-binding sites, 38 (25%) overlap with the H-NS-binding sites.