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. Author manuscript; available in PMC: 2014 Mar 8.
Published in final edited form as: Nanomedicine. 2013 Aug 27;10(2):451–461. doi: 10.1016/j.nano.2013.07.019

Figure 3.

Figure 3

PDN-disassembly as a function of pH and solvent hydrophobicity. (A) 15% native polyacrylamide gel electrophoresis of DNA filtrates released upon incubation at pH 7.4, 11.4, 5.1 and at 20%, 40% acetonitrile containing mixture. A solution of 2.5 μM d(GT)20 was included as standard. (B) Representative graphs of 260 nm UV absorbance of DNA filtrates as mentioned above. A solution of 1.275 μM d(GT)20 was included as representative of total DNA content of the PDN suspension calculated from ratiometric quantification. (C) Confocal microscopy images evaluating endosomal uptake of PDN. Cells were co-treated with PDN (magenta; left) and FITC labeled dextran (green; middle). Endosomal PDN uptake was demonstrated by colocalization both fluorescence in overlay with DIC image of the cell (right).