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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Eur J Immunol. 2013 Nov 15;44(2):450–459. doi: 10.1002/eji.201343477

Figure 2. stat4−/− mice are defective in generating a Th1 response during L. donovani infection.

Figure 2

Th1 cytokine production by splenocytes from L. donovani infected WT and stat4−/− BALB/c mice or WT and stat4−/− C57BL/6 mice stimulated with 20μg/ml LdAg. IFN-γ (A and C) and IL-12p70 (B and D) were measured by ELISA. Data shown were the mean ± SE of triplicates from four or five individual mice per group and are representative of three individual experiments. *, P < 0.05 and **, P < 0.01 using unpaired t test.

Th1 cytokine and chemokine mRNA expression by spleen tissue from L. donovani infected WT and stat4−/− BALB/c mice or WT and stat4−/− C57BL/6 mice. IFN-γ mRNA (E and G), IL-12p35 mRNA (F and H), CXCL10 mRNA (I and K) and CCL2 mRNA (J and L) were measured by Real Time PCR. Intracellular IFN-γ production by CD4+ T cells isolated from spleens of L. donovani infected WT (M) and stat4−/− (N) mice. Average percentage of IFN-γ producing CD4+ T cells of L. donovani infected WT and stat4−/− (O) mice. (n=5 mice per group). *, P < 0.05, **, P < 0.01 and ***, P < 0.001 using unpaired t test.