Figure 6. GYY4137 treatment activates the PI3K pathway and decreases apoptosis in O₂-induced lung injury.

(A) Immunoblots show decreased P-Akt and Sirtuin1 expression in hyperoxic-exposed lungs. (B) Treatment with H₂S increased expression of P-Akt and Sirtuin1 expression in hyperoxic lungs (n = 3/group, *P<0.005). (C) In vivo H₂S decreases apoptosis in oxygen-exposed lungs with BPD. Immunoblots of total caspase-3 and actin are shown for the four experimental groups. (D) Hyperoxia-exposed lungs showed increased total caspase-3 expression, which was attenuated by in vivo H₂S treatment (n = 3/group, *P<0.005).