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. 2014 Mar 6;9(3):e90965. doi: 10.1371/journal.pone.0090965

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© 2014 Vadivel et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Representative confocal microscopy at high magnification (×100) of rat lung epithelial cells (RLEs) showing (A) decreased ΔΨm (TMRM) and (C) increased mROS production (MitoSOX) in hyperoxia (TMRM and MitoSOX are in red, merged with nuclear stain DAPI in blue). Hyperoxia exposed RLEs treated with H₂S have significantly increased ΔΨm (B) and decreased mROS (D) compared to hyperoxic control (n = 4 per group, *P<0.005 hyperoxia vs. other groups).