Table 2.

NADH turnover rates

P450 type	Redox partner	NADH turnover (min−1)	Coupling efficiency (%)
WT CYP101D1	Arr/Arx	950 ± 42	95
T260A CYP101D1	Arr/Arx	522 ± 20	NA
D259N CYP101D1	Arr/Arx	NA	NA
WT CYP101D1	Pdr/Pdx	40 ± 12	69
WT P450cam	Pdr/Pdx	930 ± 28	95
WT P450cam	Arr/Arx	NA	NA

The reaction mixture contained 0.5 μM Arr, 5 μM Arx, and 0.5 μM P450 in 50 mM Tris, pH 7.4 The value for P450cam are taken from our previous work.¹² The rate of NADH oxidation for CYP101D1 in presence of either 200 μM or 1mM D-camphor at room temperature was measured by monitoring the absorbance change at 340 nm using 6.22 mM⁻¹ cm⁻¹. No significant difference in rates were observed at the two camphor concentrations. NA means no significant turnover or product formation was observed.