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. 2014 Mar 6;9(3):e90945. doi: 10.1371/journal.pone.0090945

Figure 1. PCR-RFLP for the modifier genes analysis.

Figure 1

1A: the 134 bp PCR product from exon 1 of the MBL1 gene was digested with Mwo I, Ban I and Mbo II for detection of polymorphisms in the 52, 54 and 57 codons. 1B: the 816 bp PCR product from promoter region of the IL-8 gene was digested with Mfe I for detection of the −251 polymorphism. 1C: the 142 bp PCR product from promoter region of the TNFα gene was digested with Nco I for detection of the −308 polymorphism (TNF2); the 98 bp PCR product from the SERPINA1 gene was digested with Taq Iα for detection of the S genetic variant; the 144 bp PCR product from the SERPINA1 gene was digested with Taq Iα enzyme for detection of the Z genetic variant. Mw1 is the molecular marker pBs+Msp I, Mw2 is the molecular marker λ+Pst I. PMBL, PIL8, PTNF, PAATS and PAATZ are undigested PCR products. The Z allele was not detected.