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. 2014 Mar 6;9(3):e90887. doi: 10.1371/journal.pone.0090887

Figure 3. Percentages of spermatozoa with intact plasma membrane evaluated through PNA-FITC/PI assay.

Figure 3

Bars show percentages of PNA-FITC−/PI− spermatozoa (as mean±SEM) before and after freeze/thawing when sperm is stored at 17°C either for 3 h or for 24 h. Different superscripts (a, b, c, d) mean significant differences (P<0.05) among bars, each bar representing different holding times (3 h or 24 h), preservation (Ext vs. F-T) and post-thawing times (30 min or 240 min). Ext = Extended semen (17°C). FT-C = Frozen-thawed semen.