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. 2014 Mar 6;9(3):e90887. doi: 10.1371/journal.pone.0090887

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© 2014 Yeste et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The figure shows percentages (mean±SEM) of viable spermatozoa with low membrane lipid disorder (M540^−/YO-PRO-1⁻) before and after freeze/thawing when sperm is stored at 17°C either for 3 h or for 24 h. Different superscripts (a, b, c, d, e) mean significant differences (P<0.05) among bars, each bar representing different holding times (3 h or 24 h), preservation (Ext vs. F-T) and post-thawing times (30 min or 240 min). Ext = Extended semen (17°C). FT-C = Frozen-thawed semen.