Figure 7. Intracellular peroxide levels (H₂DCFDA/PI staining), as percentages (as mean±SEM) of viable spermatozoa with high levels of intracellular peroxides (DCF ⁺/PI⁻) before and after freeze/thawing when sperm is stored at 17°C either for 3 h or for 24 h.

Different superscripts (a, b, c, d, e) mean significant differences (P<0.05) among bars, each bar representing different holding times (3 h or 24 h), preservation (Ext vs. F-T) and post-thawing times (30 min or 240 min). Ext = Extended semen (17°C). FT-C = Frozen-thawed semen.