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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Clin Immunol. 2013 Nov 27;150(2):153–160. doi: 10.1016/j.clim.2013.11.010

Figure 2. Systemic adaptive immune responses to VV are intact in IL-10−/− mice.

Figure 2

IL-10−/− and WT mice were VV-infected via skin scarification with 1×107 PFU. Single-cell suspensions of splenocytes were prepared 7 days later and fluorescently stained to identify the number of cells A) CD8+ and VV-pentamer+ and B) CD8+ and IFNγ+ (3–6 mice per group). C) Splenocytes were cultured at 106 cells/ml, and release of IFNγ, IL-6, IL-17, and IL-4 into the cell medium was measured by ELISA after 72 hours in culture (3–9 mice per uninfected group, 5–19 mice per infected group, mean ± SEM). * p < 0.05, ***p < 0.0005, by unpaired t test, two-tailed.