Table 1. Sequences of Primer and Probes used for real-time PCR (TaqMan).
Target | Forward Primer | Reverse Primer | Probe |
NAMPT | GCA GAA GCC GAG TTC AAC ATC | TGC TTG TGT TGG GTG GAT ATT G | TGG CCA CCG ACT CCT ACA AGG TTA CTC AC |
beta-ACTIN | CGA GCG CGG CTA CAG CTT | CCT TAA TGT CAC GCA CGA TTT | ACC ACC ACG GCC GAG CGG |
TBP | TTG TAA ACT TGA CCT AAA GAC CAT TGC | TTC GTG GCT CTC TTA TCC TCA TG | AAC GCC GAA TAT AAT CCC AAG CGG TTT G |
HPRT | GGC AGT ATA ATC CAA AGA TGG TCA A | GTC TGG CTT ATA TCC AAC ACT TCG T | CAA GCT TGC TGG TGA AAA GGA CCC C |
p21 | CGAAGTCAGTTCCTTGTGGAG | CATGGGTTCTGACGGACAT | – |
NAMPT (nicotinamide phosphoribosyltransferase, also known as PBEF, visfatin); p21; housekeeping genes beta-ACTIN, TBP (TATA-box-binding protein) and HPRT (hypoxanthine phophoribosyltransferase).