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. 2014 Mar 6;9(3):e90850. doi: 10.1371/journal.pone.0090850

Figure 1. Protective effects of berberine (BBR) in PC12 cells subjected to oxygen and glucose deprivation (OGD).

Figure 1

(A) Protective effect of different concentrations of BBR in PC12 cells after OGD as indicated by MTT assay. (B)–(C) Anti-apoptotic effects of BBR in PC12 cells subjected to OGD as indicated using Annexin V-FITC double staining and flow cytometry. (D)–(E) Effects of BBR on cell cycle arrest in PC12 cells subjected to OGD as indicated by flow cytometry. (F) The cell growth curves with and without BBR in different days. The final concentration of BBR was 0.5 µg/ml. BBR was added to the culture 1 h before OGD treatment and throughout reperfusion. # P<0.05 v.s. the control, ## P<0.01 v.s. the control; *P<0.05, **<0.01 v.s. the OGD. Data are presented as mean ± S.D. of six independent experiments (n = 6).