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. 2014 Mar 6;9(3):e90850. doi: 10.1371/journal.pone.0090850

Figure 2. Effect of berberine (BBR) on the cell cycles and Rb expressions in PC12 cells during the 6 h of reperfusion after 2 h of OGD.

Figure 2

(A) Expression of Rb proteins and time schedule of the experiment. (B) The percentage of cells in the G1 phase. (C) Total Rb protein. (D) Ratio of phosphor-Rb (S795) to total Rb (phosphor-Rb/Rb). Berberine (0.5 µg/ml) was added to the culture 1 h before OGD treatment and throughout reperfusion. Data are presented as mean ± S.D. of three independent experiments (n = 3).