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. 2014 Mar 6;9(3):e90850. doi: 10.1371/journal.pone.0090850

Figure 5. Effects of berberine (BBR) on the PC12 cells with RB1 knockdown.

Figure 5

(A) Expression of Rb in normal and RB1 knockdown cells. RB1shRNA was constructed to lentiviral vector PLL3.7. (B) Cell viabilities of normal and RB1 knockdown cells. Columns represent the mean ± S.D. ## P<0.01 v.s. control groups; *P<0.05 v.s. model groups (n = 6). (C) Phases of cell cycle were detected in normal and RB1 knockdown cells. (D) Apoptosis of BBR after OGD treated cells using Annexin V-FITC double staining were detected by using flow cytometry. Data are presented as mean ± S.D. ## P<0.01 v.s. control groups; *P<0.05, **P<0.01 v.s. the OGD (n = 6). (E)–(F) Expression of Rb in cells infected with carrying RB1 shRNAlentivirus under normal and OGD conditions. BBR (0.5 µg/ml) was added in the culture 1 h before OGD treatment and throughout reperfusion. Data are presented as mean ± S.D. of three independent experiments (n = 3). ## P<0.01 v.s. the normal; **P<0.01 v.s. the OGD.