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. 2014 Mar 6;10(3):e1003936. doi: 10.1371/journal.ppat.1003936

Figure 1. GAG inhibits Aspergillus-induced human T-helper cell cytokine production.

Figure 1

(A) TNFα, IL-6, IL-8 and IL-10 concentrations in culture supernatants of human PBMCs stimulated for 24 hours with 10 µg/ml GAG and IFN-γ, IL-17, IL-5 and IL-9 concentrations after 7 days of stimulation. (B) TNFα, IL-6 and IL-10 concentrations in culture supernatants of human PBMCs (n = 6 donors) stimulated for 24 hours with heat inactivated A. fumigatus conidia (1×107/ml) in the presence or absence of 10 µg/ml GAG. (C,D) IL-17, IL-22 and IFN-γ concentrations in culture supernatants of human PBMCs stimulated for 7 days with heat inactivated A. fumigatus conidia (1×107/ml) (n = 10 donors for IL-17 and IL-22, n = 6 donors for IFN-γ) (c), IL-1β/IL-23 (50/100 ng/ml) (n = 14 donors) or IL-12/IL-18 (50/100 ng/ml) (n = 10 donors) in the presence or absence of GAG (10 µg/ml). Data are represented as mean +/− SEM.