Table 2. Expression of T. denticola genes encoding enzymes involved in glycine or glycine-related metabolism during co-culture with P. gingivalis.
Protein function(s) | Gene | Expression fold change (co-culture versus mono-culture) | ||
Microarray | qRT-PCR | |||
Glycine cleavage | TDE1624 P-protein subunit 2 | 1.71 | 1.81 | |
TDE1625 P-protein subunit 1 | 1.51 | 1.61 | ||
TDE1626 H-protein | 1.41 | 1.71 | ||
TDE1627 T-protein | 1.31 | 1.81 | ||
TDE1629 L-protein | 1.31 | ND | ||
Conversion of methylene THF to 10-formylTHF | TDE0013 MethyleneTHF dehydrogenase/MethenylTHF cyclohydrolase | 1.51 | ND | |
Conversion of 10-formylTHF to formate | TDE0019 FormylTHF synthase | 1.0 | ND | |
Glycine reductase system | Protein B1 | TDE0078 GrdB1 | −1.31 | ND |
TDE0077 GrdE1 | −1.21 | ND | ||
Protein B2 | TDE2119 GrdB2 | 1.41 | 2.11 | |
TDE2120 GrdE2 | 1.31 | 1.71 | ||
Protein A | TDE0745 GrdA | −1.1 | ND2 | |
Protein C | TDE0240 GrdC | −1.1 | ND | |
TDE0239 GrdD | −1.1 | ND | ||
Conversion of acetyl-phosphate to acetate | TDE0933 Acetate kinase | 1.21 | ND | |
Alanine/Glycine cation symporter (AGCS) | TDE1259 Na+/Alanine-glycine symporter | 1.41 | 1.81 |
represents fold change with adjusted p<0.05.
Not determined.