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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Cancer Res. 2013 Nov 27;74(3):908–920. doi: 10.1158/0008-5472.CAN-13-2034

Figure 7.

Figure 7

MCT1 Inhibitor/metformin combination triggers increases in hydrogen peroxide, mitochondrial damage and cell death that is blocked by glutathione. Raji cells were treated with vehicle, SR13800 (1 µM), metformin (1 mM) or SR13800 plus metformin for the indicated times and analyzed by flow cytometry for: A, DCFDA; and B, Mitotracker Red stain (representative of three experiments). C–F, NAC (C, D) and GSH (E, F) override the anti-cancer effects of SR13800 +/− metformin. Raji cells were pretreated with or without NAC (10 mM) or glutathione ester (3 mM [D] or 5 mM [E, F]) for 1 hr and then treated with vehicle or SR13800 (100 nM [C–E] or 1 µM [F]) +/− metformin (1 mM). C, MTT assays after 3 days (n = 3). D, E, Cell survival by trypan blue dye exclusion (n = 3). F, DCFDA fluorescence after 24 hr of treatment (n = 3).