Figure 5. Western blots for DBC2-associated components from reticulocyte lysates identified by LC-MS/MS.
Flag-tagged DBC2 was synthesized and immuno-adsorbed from RRL with anti-Flag antibody-agarose, washed with P100T, and analyzed by SDS-PAGE, and Western blotting for DBC2 and co-adsorption of Cul3 and CSN4 as described under Materials and methods.